Electron Transfer between Azurin from Alcaligenes faecalis and Cytochrome c551 from Pseudomonas aeruginosa
نویسندگان
چکیده
منابع مشابه
The electron-transfer reaction between azurin and the cytochrome c oxidase from Pseudomonas aeruginosa.
A stopped-flow investigation of the electron-transfer reaction between oxidized azurin and reduced Pseudomonas aeruginosa cytochrome c-551 oxidase and between reduced azurin and oxidized Ps. aeruginosa cytochrome c-551 oxidase was performed. Electrons leave and enter the oxidase molecule via its haem c component, with the oxidation and reduction of the haem d1 occurring by internal electron tra...
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Type zero copper is a hard-ligand analogue of the classical type 1 or blue site in copper proteins that function as electron transfer (ET) agents in photosynthesis and other biological processes. The EPR spectroscopic features of type zero Cu(II) are very similar to those of blue copper, although lacking the deep blue color, due to the absence of thiolate ligation. We have measured the rates of...
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Unfolding pathways of Pseudomonas aeruginosa cytochrome c551 (Pa cyt c) characterized by native state hydrogen-deuterium exchange (NS H/D) methods in conjunction with multi-dimensional NMR techniques have been reported in the literature. Based on the analyses carried out on the H/D exchange data, it has been shown that there are four submolecular unfolding units or three cryptic intermediates (...
متن کاملDynamic effects of mutations within two loops of cytochrome c551 from Pseudomonas aeruginosa.
In this work, we investigated the structural and dynamic consequences of two substitutions, P58A and G36P, located in two different solvent-exposed loops of cytochrome c551. The results show that both mutations affect regions that are distant from the site of mutation. Here, the two loops appear to be dynamically coupled to each other, because the substitution at one site affects the structure ...
متن کاملCytochrome c550 from Pseudomonas aeruginosa.
In cells of Pseudomonas aeruginosa A.T.C.C. 17933 grown on ethanol the synthesis of a soluble c-type cytochrome, together with quinoprotein ethanol dehydrogenase, is induced. The cytochrome, with an alpha-absorption band at 550 nm, was purified to homogeneity. The molecular mass of the monomeric protein is 15 kDa, the pI is 4.8, and it contains one haem prosthetic group. The midpoint potential ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1981
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1981.tb05709.x